The educator at Ace Pathshala, Dr. Pooja, is an accomplished biologist who holds a PhD in Neuroendocrinology from the University of Delhi, India. In addition to her PhD, she also holds M.Phil., MSc, and BSc degrees in Zoology from the same institution, where she was recognized for her outstanding academic achievements with a Gold medal during her undergraduate studies.
Dr. Pooja has extensive teaching experience at various coaching institutes, specifically for highly competitive exams such as CSIR-NET, GATE, ICMR, IISC, ICAR, IIT-JAM, TIFR, BARC, NEET, MSc, CUET-PG, CUET-UG, BSc, PhD entrances, UPSC Zoology Optional, and others. As an assistant professor at Panjab University College, she has also mentored numerous students and facilitated their academic growth.
Through Ace Pathshala, Dr. Pooja aims to share her extensive knowledge and experience with enthusiastic biology learners, ensuring that their learning is both accessible and convenient. Her approach to teaching is characterized by clarity, engagement, and attention to detail. Dr. Pooja is passionate about helping students achieve their academic goals and empowering them to pursue their interests in the field of biology.
Kisspeptin, Dopamine and GnRH regulate gonadotropin release and oocyte maturation: in vivo, in vitro and in silico analyses in the catfish, Heteropneustes fossilis (Bloch).
“Effect of estradiol 17- β on apoptosis and vitellogenin synthesis in the African catfish, Clarias gariepinus.’’
‘Effect of estrogen exposure on early life stages of Zebra fish, Danio rerio.”
Molecular techniques like RNA and DNA isolation, cDNA preparation, PCR (Polymerase Chain Reaction), Real Time PCR, Cloning and related techniques like transformation, plasmid purification, In situ hybridisation, SEM and TEM
Cell Culture: Pituitary culture, Hepatocyte culture, Oocyte culture, Theca and Granulosa cell culture
Electrophoretic techniques: Agarose gel electrophoresis, SDS PAGE, Gel density analysis and quantification with ImageJ software,
Immunological Techniques: ELISA, Western blotting, Immunohistochemistry, ODD (Ouchterlony double diffusion), Immunoglobulin G purification by Affinity chromatography
Biochemical techniques: Protein estimation, Sugar estimation, Protein Purification: Precipitation, Dialysis, Column Chromatography, Biochemical testing of various compound
Histochemical Staining: Hematoxylin-eosin, Cresyl violet, Mallory trichrome
Instrument Handling: Microscopy, UV/Visible Spectrophotometer, Transilluminator, Centrifuge, Thermal cycler, Laminar Air Flow, Electrophoresis system, HPLC, FPLC, Confocal Microscope, Real time PCR, Fluorescent microscope, Microtome, Cryotome.
Bioinformatics skills: Searching Tools & Databases: NCBI, Similarity Searching Tools: BLAST, FASTA, Sequence Alignment and Phylogenetic Analysis Tools: Clustal W, PHYLIP and MEGA, Proficient with Microsoft office.
In silico protein prediction and docking of proteins: Modeller, I-TASSER, Swiss-modelling, Haddock, PROCHECK, ERRAT, ProSA and many more.
Developed e-resources for postgraduate course entitled Module- Methods for gene identification: Cloning (M-23) (Paper P16-Molecular Genetics) for National Mission on Education Information Communication Technology (NME-ICT) in Zoology under MHRD Project, Government of India.
http://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83aA==
Developed e-resources for postgraduate course entitled Module- Methods for gene identification: Southern Blot Hybridization (M-22) (Paper P16-Molecular Genetics) for National Mission on Education Information Communication Technology (NME-ICT) in Zoology under MHRD Project, Government of India.
http://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83aA==
Developed e-resources for postgraduate course entitled Module- Gene knock out and Gene knock down (Model Organisms) (M-18) (Paper P16-Molecular Genetics) for National Mission on Education Information Communication Technology (NME-ICT) in Zoology under MHRD Project, Government of India.
http://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83aA==
Developed e-resources for postgraduate course entitled Methods for gene identification: Polymerase Chain Reaction (M-24) (Paper P16-Molecular Genetics) for National Mission on Education Information Communication Technology (NME-ICT) in Zoology under MHRD Project, Government of India.
http://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83aA==
Developed e-resources for postgraduate course entitled Module- Cytoskeleton (M-13) (Paper P15-Molecular Cell Biology) for National Mission on Education Information Communication Technology (NME-ICT) in Zoology under MHRD Project, Government of India.
http://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83aA==
Journal of Biomolecular Structure and Dynamics (Taylor & Francis), Animal Reproduction Science (Elsevier), Journal of Applied and Natural Science, Iranian Journal of Fisheries Sciences, Journal of Pharmaceutical Research International, The Applied Biology & Chemistry Journal, International Journal of Applied Chemical and Biological Sciences, etc.
Research gate: https://www.researchgate.net/profile/Pooja-Kumari-15
Publons ID: https://publons.com/researcher/4639949/pooja-kumari/peer-review/
Orcid ID: https://orcid.org/0000-0002-1155-3044
Web of science researcher ID: AAW-7629-2021